Rotovap machine is commonly used for compound extraction, concentration, and purification. Our rotary steamer has large-scale rotary evaporator and a small rotary evaporator. Here’s a general step-by-step guide on how to use a rotovap for extraction:
Note: Always follow the manufacturer’s instructions and any specific protocols provided by your lab or institution when using a rotary evaporator.
Materials Needed:
Rotary Evaporator
Sample in a solvent
Vacuum pump
Cooling chiller
Flask for collecting the solvent
Extraction steps:
Set Up the Apparatus:
This involves attaching the flask, condenser, heating bath, vacuum pump, and other components. Ensure that all connections are secure and the apparatus is appropriately sealed.
Prepare Your Sample:
Place your sample solution in a round-bottom flask. The flask is usually attached to the rotary evaporator’s central unit.
Attach the Condenser:
Connect the condenser to the central unit. The condenser is responsible for cooling the vaporized solvent, causing it to condense back into liquid form and collect in a separate flask.
Connect the Vacuum Pump:
Connect the vacuum pump to the rotary evaporator. The vacuum pump creates a low-pressure environment that facilitates evaporation by lowering the solvent’s boiling point.
Start the Heating Bath:
Fill the heating or water bath with a suitable heat transfer fluid (usually water) and set it to the desired temperature. The heating bath indirectly heats the sample flask, causing the solvent to evaporate.
Turn On the Vacuum Pump:
Turn on the vacuum pump to start creating a vacuum within the system. This reduces the pressure inside the sample flask, lowering the solvent’s boiling point and aiding in its evaporation.
Begin Rotation:
Start the rotation of the sample flask. The rotation helps to increase the surface area of the liquid, facilitating faster and more even evaporation.
Monitor the Process:
As the solvent evaporates, vapor rises from the sample flask and moves into the condenser. The solvent will condense in the condenser and drip into a separate collection flask.
Collect the Solvent:
Once the desired amount of solvent has been collected, turn off the heating bath and the vacuum pump.
Carefully detach the collection flask containing the concentrated solvent.
Release the Vacuum:
Vent the system to atmospheric pressure by gradually introducing air into the system.
How to clean rotovap after work
Shutdown and Disconnect:
Ensure the rotovap is turned off and disconnected from power sources and vacuum pumps.
Disassemble Components:
Carefully disassemble removable parts such as the flask, evaporating flask adapter, condenser, and collection flask.
If applicable, remove the sealing gaskets, O-rings, and other components that can be removed.
Remove Residues:
Wipe off any visible residues, spills, or deposits from the exterior and interior surfaces of the rotovap using a clean, damp cloth.
You can use a mild detergent solution or a suitable cleaning agent if there are stubborn residues. However, avoid using corrosive or abrasive substances that could damage the equipment.
Clean Glassware:
Clean the glass components (flasks, condenser, etc.) with warm water. If necessary, use a suitable glassware brush to remove residues.
If residues don’t come off quickly, you can soak the glass components in warm water and mild detergent. Rinse thoroughly afterward.
Cleaning the Heating Bath:
If the rotovap has a heating bath, clean its interior with a cloth dampened with isopropanol or ethanol. Avoid letting liquids get into the electrical components.
If the heating bath has stubborn residues, consult the manufacturer’s instructions for appropriate cleaning methods.
Cleaning Seals and Gaskets:
Clean the sealing gaskets and O-rings with a cloth dampened with isopropanol or ethanol to remove dirt and debris. Inspect them for signs of wear or damage; replace them with new ones if necessary.
Dry and Reassemble:
After cleaning, allow all components to air dry completely. Using lint-free cloths can help prevent lint or fibers from adhering to the equipment.
Once dry, reassemble the rotovap components, ensuring proper alignment and placement of seals and gaskets.
Final Inspection:
Inspect the cleaned rotovap for any missed residues or areas that need further attention.
Dispose of Waste Properly:
Dispose of any cleaning solutions and waste materials following your institution’s waste disposal guidelines and local regulations.